2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 አንጻራዊ የሞለኪውላር የጅምላ ስርጭትን በሚለካበት ኩርባ ውስጥ ጥቅም ላይ የዋሉ መደበኛ ንጥረ ነገሮች፡ ኢንሱሊን፣ ማይኮፔፕታይዶች፣ ግሊሲን-ግሊሲን-ታይሮሲን-አርጊኒን፣ ግሊሲን-ግሊሲን-ግሊሲን
3 መሳሪያዎች እና መሳሪያዎች
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
በአጠቃላይ፣ በሱስታር ምርቶች ውስጥ ያለው የአሚኖ አሲዶች መጠን ከዚንፕሮ ምርቶች ውስጥ ካለው ከፍተኛ ነው።
ክፍል 8 የአጠቃቀም ውጤቶች
የተለያዩ የዱቄት ማዕድናት ምንጮች በእንቁላል ጥራት እና በእንቁላል ጥራት ላይ የሚያሳድሩት ተጽዕኖ በመዝራት መጨረሻ ላይ
የምርት ሂደት
የታለመ የቼልቴሽን ቴክኖሎጂ
የሼር ኢሙልሲፊኬሽን ቴክኖሎጂ
የግፊት ስፕሬይ እና ማድረቂያ ቴክኖሎጂ
የማቀዝቀዣ እና የእርጥበት ማስወገጃ ቴክኖሎጂ
የላቀ የአካባቢ ቁጥጥር ቴክኖሎጂ
አባሪ ሀ፡ የፔፕታይዶችን አንጻራዊ ሞለኪውላዊ የጅምላ ስርጭት ለመወሰን የሚረዱ ዘዴዎች
የመደበኛ ተቀባይነት፡ GB/T 22492-2008
1 የሙከራ መርህ፡
በከፍተኛ አፈጻጸም የጄል ማጣሪያ ክሮማቶግራፊ ተወስኗል። ይህም ማለት፣ ለመለያየት የናሙና ክፍሎች አንጻራዊ ሞለኪውላዊ የጅምላ መጠን ልዩነት ላይ በመመስረት፣ በ220nm የአልትራቫዮሌት መምጠጥ ሞገድ ርዝመት ላይ የፔፕታይድ ትስስር ላይ የተገኘውን ቀዳዳ መሙያ እንደ ቋሚ ምዕራፍ በመጠቀም፣ በጄል ማጣሪያ ክሮማቶግራፊ (ማለትም የጂፒሲ ሶፍትዌር) አንጻራዊ ሞለኪውላዊ የጅምላ ስርጭትን ለመወሰን የተወሰነውን የውሂብ ማቀነባበሪያ ሶፍትዌር በመጠቀም፣ ክሮማቶግራሞቹ እና መረጃዎቻቸው ተሰርተዋል፣ የአኩሪ አተር ፔፕታይድን አንጻራዊ ሞለኪውላዊ ክብደት መጠን እና የስርጭት ክልል መጠን ለማግኘት ተሰልተዋል።
2. ሬጀንቶች
የሙከራው ውሃ በ GB/T6682 ውስጥ ያለውን ሁለተኛ ደረጃ ውሃ መስፈርት ማሟላት አለበት፣ ከልዩ ድንጋጌዎች በስተቀር የሪአጀንቶች አጠቃቀም በትንታኔያዊ መልኩ ንፁህ ነው።
2.1 ሬጀንቶች አሴቶኒትሪል (በክሮማቶግራፊያዊ መልኩ ንፁህ)፣ ትሪፍሎሮአሴቲክ አሲድ (በክሮማቶግራፊያዊ መልኩ ንፁህ)፣
2.2 አንጻራዊ የሞለኪውላር የጅምላ ስርጭትን በሚለካበት ኩርባ ውስጥ ጥቅም ላይ የዋሉ መደበኛ ንጥረ ነገሮች፡ ኢንሱሊን፣ ማይኮፔፕታይዶች፣ ግሊሲን-ግሊሲን-ታይሮሲን-አርጊኒን፣ ግሊሲን-ግሊሲን-ግሊሲን
3 መሳሪያዎች እና መሳሪያዎች
3.1 ከፍተኛ አፈጻጸም ያለው ፈሳሽ ክሮማቶግራፍ (HPLC)፡- የ UV ዳሳሽ እና የጂፒሲ የውሂብ ማቀነባበሪያ ሶፍትዌር ያለው ክሮማቶግራፊክ የስራ ጣቢያ ወይም ኢንተግሬተር።
3.2 የሞባይል ፌዝ የቫክዩም ማጣሪያ እና የጋዝ ማስወገጃ ክፍል።
3.3 የኤሌክትሮኒክ ቀሪ ሂሳብ፡ የተመረቀ እሴት 0.000 1 ግ።
4 የአሠራር ደረጃዎች
4.1 የክሮማቶግራፊክ ሁኔታዎች እና የስርዓት መላመድ ሙከራዎች (የማጣቀሻ ሁኔታዎች)
- 4.1.1 የክሮማቶግራፊክ አምድ፡ TSKgelG2000swxl300 ሚሜ × 7.8 ሚሜ (ውስጣዊ ዲያሜትር) ወይም ተመሳሳይ አይነት ጄል አምዶች እና ፕሮቲኖችን እና ፔፕታይዶችን ለመወሰን ተስማሚ ናቸው።
- 4.1.2 የሞባይል ደረጃ፡ አሴቶኒትሪል + ውሃ + ትራይፍሎሮአሴቲክ አሲድ = 20 + 80 + 0.1።
- 4.1.3 የመለኪያ የሞገድ ርዝመት፡ 220 nm።
- 4.1.4 የፍሰት መጠን፡ 0.5 ሚሊ ሊትር/ደቂቃ።
- 4.1.5 የመለየት ጊዜ፡ 30 ደቂቃ።
- 4.1.6 የናሙና መርፌ መጠን፡ 20μL።
- 4.1.7 የአምድ ሙቀት፡ የክፍል ሙቀት።
- 4.1.8 የክሮማቶግራፊ ስርዓቱ የመለየት መስፈርቶችን እንዲያሟላ ለማድረግ፣ ከላይ በተጠቀሱት የክሮማቶግራፊክ ሁኔታዎች ስር፣ የጄል ክሮማቶግራፊክ አምድ ቅልጥፍና፣ ማለትም የቲዎሬቲካል ሳህኖች ብዛት (N)፣ በትሪፔፕታይድ ደረጃ (ግሊሲን-ግሊሲን-ግሊሲን) ጫፎች ላይ በመመስረት ከ10000 ያላነሰ መሆኑን ተደንግጓል።
- 4.2 አንጻራዊ የሞለኪውላር ክብደት መደበኛ ኩርባዎች ማምረት
- ከላይ የተጠቀሱት የተለያዩ አንጻራዊ ሞለኪውላዊ የጅምላ ፔፕታይድ መደበኛ መፍትሄዎች 1 mg / mL የጅምላ ክምችት ያላቸው በሞባይል የደረጃ ማመሳሰል ተዘጋጅተው በተወሰነ መጠን ተቀላቅለው ከዚያም 0.2 μm ~ 0.5 μm የሆነ የቀዳዳ መጠን ባለው ኦርጋኒክ የደረጃ ሽፋን በኩል ተጣርተው ወደ ናሙናው ውስጥ ገብተዋል፣ ከዚያም የመመዘኛዎቹ ክሮማቶግራሞች ተገኝተዋል። አንጻራዊ ሞለኪውላዊ የጅምላ መለኪያ ኩርባዎች እና እኩልታዎቻቸው የተገኙት አንጻራዊ ሞለኪውላዊ የጅምላ ሎጋሪዝምን ከማቆያ ጊዜ ጋር በማነፃፀር ወይም በመስመራዊ ሪግሬሽን በማስቀመጥ ነው።
4.3 የናሙና ህክምና
10 ሚ.ግ ናሙናውን በ10 ሚሊ ሊትር ቮልሜትሪክ ብልቃጥ ውስጥ በትክክል ይመዝኑ፣ ትንሽ ተንቀሳቃሽ ፎዝ ይጨምሩ፣ ለ10 ደቂቃ የአልትራሳውንድ መንቀጥቀጥ ይጨምሩበት፣ በዚህም ናሙናው ሙሉ በሙሉ ይሟሟል እና ይቀላቀላል፣ ከሞባይል ፎዝ ጋር ወደ ሚዛን ይቀልጣል፣ ከዚያም 0.2 μm ~ 0.5 μm የሆነ ቀዳዳ ባለው ኦርጋኒክ ፎዝ ሽፋን ውስጥ ይጣራል፣ እና ማጣሪያው በA.4.1 ውስጥ ባለው የክሮሞቶግራፊ ሁኔታ መሰረት ተተነተነ።
- 5. አንጻራዊ የሞለኪውላር ክብደት ስርጭት ስሌት
- በ4.1 ክሮማቶግራፊያዊ ሁኔታዎች ስር በ4.3 የተዘጋጀውን የናሙና መፍትሄ ከተመረመረ በኋላ፣ የናሙናው አንጻራዊ ሞለኪውላዊ ክብደት እና የስርጭት ክልሉ የናሙናውን የክሮማቶግራፊ መረጃ ወደ የካሊብሬሽን ኩርባ 4.2 በጂፒሲ የውሂብ ማቀነባበሪያ ሶፍትዌር በመተካት ማግኘት ይቻላል። የተለያዩ የፔፕታይዶች አንጻራዊ ሞለኪውላዊ ክብደት ስርጭት በቀመር መሠረት በፒክ አካባቢ መደበኛነት ዘዴ ሊሰላ ይችላል፡ X=A/A ጠቅላላ×100
- በቀመር ውስጥ፡ X - በናሙናው ውስጥ ባለው አጠቃላይ ፔፕታይድ ውስጥ የአንድ አንጻራዊ ሞለኪውላዊ ክብደት ፔፕታይድ የጅምላ ክፍልፋይ፣ %፤
- ሀ - አንጻራዊ የሞለኪውላር ክብደት ፔፕታይድ ጫፍ አካባቢ፤
- ጠቅላላ ሀ - የእያንዳንዱ አንጻራዊ ሞለኪውላዊ ክብደት ፔፕታይድ ከፍተኛ ቦታዎች ድምር፣ ወደ አንድ የአስርዮሽ ቦታ ይሰላል።
- 6 ተደጋጋሚነት
- በተደጋጋሚነት ሁኔታዎች ስር የተገኙ ሁለት ገለልተኛ ውሳኔዎች መካከል ያለው ፍጹም ልዩነት ከሁለቱ ውሳኔዎች የሂሳብ አማካይ 15% መብለጥ የለበትም።
- አባሪ ለ፡ ነፃ አሚኖ አሲዶችን ለመለየት የሚረዱ ዘዴዎች
- የደረጃ ተቀባይነት፡ Q/320205 KAVN05-2016
- 1.2 ሬጀንቶች እና ቁሳቁሶች
- ግላሻል አሴቲክ አሲድ፡- በትንታኔ ንፁህ
- ፐርክሎሪክ አሲድ፡ 0.0500 ሞል/ሊ
- አመልካች፡ 0.1% የክሪስታል ቫዮሌት አመልካች (ግላሲያል አሴቲክ አሲድ)
- 2. ነፃ የአሚኖ አሲዶችን መወሰን
ናሙናዎቹ በ80°ሴ ለ1 ሰዓት ደርቀዋል።
ናሙናውን በተፈጥሮ ወደ ክፍል የሙቀት መጠን እንዲቀዘቅዝ ወይም ጥቅም ላይ ሊውል ወደሚችል የሙቀት መጠን እንዲቀዘቅዝ በደረቅ መያዣ ውስጥ ያስቀምጡት።በግምት 0.1 ግራም የናሙና ናሙና (እስከ 0.001 ግራም) በ250 ሚሊ ሊትር ደረቅ ሾጣጣ ብልቃጥ ውስጥ ይመዝኑ።ናሙናው የአካባቢውን እርጥበት እንዳይስብ በፍጥነት ወደሚቀጥለው ደረጃ ይሂዱ25 ሚሊ ሊትር የግላሲያል አሲቲክ አሲድ ይጨምሩ እና ከ 5 ደቂቃዎች በማይበልጥ ጊዜ ውስጥ በደንብ ይቀላቅሉ።2 የክሪስታል ቫዮሌት አመልካች ጠብታዎችን ያክሉመፍትሄው ከሐምራዊ እስከ መጨረሻው ነጥብ እስኪለወጥ ድረስ በ0.0500 mol / L (±0.001) መደበኛ የፔርክሎሪክ አሲድ የቲትሬሽን መፍትሄ ቲትሬት ያድርጉ።
የተጠቀመውን መደበኛ መፍትሄ መጠን ይመዝግቡ።
- ባዶውን ምርመራ በተመሳሳይ ጊዜ ያካሂዱ።
- 3. ስሌት እና ውጤቶች
- በሪአክተቱ ውስጥ ያለው ነፃ የአሚኖ አሲድ ይዘት X እንደ የጅምላ ክፍልፋይ (%) ይገለጻል እና በቀመር መሰረት ይሰላል፡ X = C × (V1-V0) × 0.1445/M × 100%፣ በ tne ፎርሙላ፡
- ሐ - በሞሎች በአንድ ሊትር (ሞል/ሊ) ውስጥ መደበኛ የፐርክሎሪክ አሲድ መፍትሄ ክምችት
- ቪ1 - በመደበኛ የፐርክሎሪክ አሲድ መፍትሄ፣ በሚሊሊየር (mL) ውስጥ ናሙናዎችን ለቲትሬሽን የሚያገለግል መጠን።
- ቮ - ለቲትሬሽን ባዶነት ከመደበኛ የፐርክሎሪክ አሲድ መፍትሄ ጋር፣ በሚሊሊየር (mL) ውስጥ ጥቅም ላይ የሚውል መጠን፤
M - የናሙናው ክብደት፣ በግራም (ግ)።
| 0.1445፡ ከ1.00 ሚሊ ሊትር መደበኛ የፐርክሎሪክ አሲድ መፍትሄ ጋር እኩል የሆነ የአሚኖ አሲዶች አማካይ ክብደት [c (HClO4) = 1.000 mol / L]። | 4.2.3 የሴሪየም ሰልፌት መደበኛ የቲቴሽን መፍትሄ፡ ክምችት c [Ce (SO4) 2] = 0.1 mol/L፣ በ GB/T601 መሰረት የተዘጋጀ። | |
| የደረጃዎች ተቀባይነት፡ Q/70920556 71-2024 | 1. የውሳኔ መርህ (Fe እንደ ምሳሌ) | የአሚኖ አሲድ የብረት ውህዶች በሃይድሮድ ኤታኖል ውስጥ በጣም ዝቅተኛ የመሟሟት አቅም አላቸው እና ነፃ የብረት አየኖች በሃይድሮድ ኤታኖል ውስጥ የሚሟሟሉ ናቸው፣ በሁለቱ ውስጥ በሃይድሮድ ኤታኖል ውስጥ ያለው የመሟሟት ልዩነት የአሚኖ አሲድ የብረት ውህዶችን የቼሌሽን መጠን ለመወሰን ጥቅም ላይ ውሏል። |
| በቀመር ውስጥ፡ V1 - ለሙከራ መፍትሄ ቲቴሬሽን የሚውል የሴሪየም ሰልፌት መደበኛ መፍትሄ መጠን፣ mL፤ | አንሃይድሮስ ኤታኖል፤ የተቀረው በGB/T 27983-2011 ውስጥ ካለው አንቀጽ 4.5.2 ጋር ተመሳሳይ ነው። | 3. የትንታኔ ደረጃዎች |
| ሁለት ሙከራዎችን በትይዩ ያድርጉ። 0.1 ግራም የናሙናውን ክብደት በ103±2℃ ለ1 ሰዓት ደርቆ፣ እስከ 0.0001 ግ ድረስ ትክክል፣ 100 ሚሊ ሊትር የማይጠጣ ኤታኖል ይጨምሩ፣ በ100 ሚሊ ሊትር የማይጠጣ ኤታኖል የታጠበውን ቅሪት ያጣሩ፣ ያጣሩ፣ ያጣሩ፣ ቢያንስ ለሦስት ጊዜ ያጣሩ፣ ከዚያም ቅሪቱን ወደ 250 ሚሊ ሊትር ሾጣጣ ብልቃጥ ያስተላልፉ፣ በGB/T27983-2011 አንቀጽ 4.5.3 መሠረት 10 ሚሊ ሊትር የሰልፈሪክ አሲድ መፍትሄ ይጨምሩ፣ እና ከዚያም በGB/T27983-2011 አንቀጽ 4.5.3 መሠረት “ለመሟሟት ያሞቁ እና ከዚያ እንዲቀዘቅዝ ያድርጉ” የሚሉትን ደረጃዎች ያከናውኑ። ባዶውን ሙከራ በተመሳሳይ ጊዜ ያካሂዱ። | 4. የጠቅላላ የብረት ይዘት መወሰን | 4.1 የውሳኔ መርህ ከ GB/T 21996-2008 አንቀጽ 4.4.1 ጋር ተመሳሳይ ነው። |
4.2. ሬጀንቶች እና መፍትሄዎች
| 4.2.1 የተቀላቀለ አሲድ፡ 150 ሚሊ ሊትር የሰልፈሪክ አሲድ እና 150 ሚሊ ሊትር የፎስፈረስ አሲድ ወደ 700 ሚሊ ሊትር ውሃ ይጨምሩ እና በደንብ ይቀላቅሉ። | 4.2.2 የሶዲየም ዲፊኒላሚን ሰልፎኔት አመልካች መፍትሄ፡ 5 ግራም/ሊ፣ በጂቢ/ቲ603 መሰረት የተዘጋጀ። | 4.2.3 የሴሪየም ሰልፌት መደበኛ የቲቴሽን መፍትሄ፡ ክምችት c [Ce (SO4) 2] = 0.1 mol/L፣ በ GB/T601 መሰረት የተዘጋጀ። | |
| 4.3 የትንታኔ ደረጃዎች | ሁለት ሙከራዎችን በትይዩ ያድርጉ። 0.1 ግራም ናሙና ይመዝኑ፣ እስከ 020001 ግራም ድረስ ትክክል፣ በ250 ሚሊ ሊትር ሾጣጣ ጠርሙስ ውስጥ ያስቀምጡ፣ 10 ሚሊ ሊትር የተቀላቀለ አሲድ ይጨምሩ፣ ከተሟሟ በኋላ፣ 30 ሚሊ ሊትር ውሃ እና 4 ጠብታዎች የሶዲየም ዲያኒሊን ሰልፎኔት አመልካች መፍትሄ ይጨምሩ፣ ከዚያም በ GB/T21996-2008 ውስጥ በአንቀጽ 4.4.2 መሠረት የሚከተሉትን ደረጃዎች ያከናውኑ። ባዶውን ምርመራ በተመሳሳይ ጊዜ ያካሂዱ። | 4.4 የውጤቶች ውክልና | የአሚኖ አሲድ የብረት ውህዶች አጠቃላይ የብረት ይዘት X1 በብረት የጅምላ ክፍልፋይ፣ በ% የተገለጸው እሴት፣ በቀመር (1) መሠረት ተሰልቷል፡ |
| X1=(V-V0)×C×M×10-3×100 | V0 - ባዶ መፍትሄን ለማጣፈጥ የሚውል የሴሪየም ሰልፌት መደበኛ መፍትሄ፣ mL፤ | V0 - ባዶ መፍትሄን ለማጣፈጥ የሚውል የሴሪየም ሰልፌት መደበኛ መፍትሄ፣ mL፤ | ሐ - የሴሪየም ሰልፌት መደበኛ መፍትሄ ትክክለኛ ክምችት፣ ሞል/ሊ5. በኬሌትስ ውስጥ የብረት ይዘት ስሌትበኬሌት ውስጥ ያለው የብረት ይዘት X2 በብረት የጅምላ ክፍልፋይ፣ በ% የተገለጸው እሴት፣ በቀመር መሰረት ተሰልቷል፡ x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| በቀመር ውስጥ፡ V1 - ለሙከራ መፍትሄ ቲቴሬሽን የሚውል የሴሪየም ሰልፌት መደበኛ መፍትሄ መጠን፣ mL፤ | ቪ2 - ባዶ መፍትሄን ለማጣፈጥ የሚውል የሴሪየም ሰልፌት መደበኛ መፍትሄ፣ mL፤nom1-የናሙናው ክብደት፣ ሰ. የትይዩ ውሳኔ ውጤቶችን የሂሳብ አማካኝ እንደ መወሰን ውጤቶች ይውሰዱ፣ እና የትይዩ ውሳኔ ውጤቶች ፍጹም ልዩነት ከ 0.3% አይበልጥም። | 0.05585 - ከ1.00 ሚሊ ሊትር የሴሪየም ሰልፌት መደበኛ መፍትሄ ጋር እኩል በሆነ ግራም የተገለጸ የብረታ ብረት ብዛት C[Ce(SO4)2.4H20] = 1.000 ሞል/ሊ።nom1-የናሙናው ክብደት፣ ሰ. የትይዩ ውሳኔ ውጤቶችን የሂሳብ አማካኝ እንደ መወሰን ውጤቶች ይውሰዱ፣ እና የትይዩ ውሳኔ ውጤቶች ፍጹም ልዩነት ከ 0.3% አይበልጥም። | 6. የኬሌሽን መጠን ስሌትየኬሌሽን መጠን X3፣ በ% የተገለጸው እሴት፣ X3 = X2/X1 × 100አባሪ ሐ፡ የዚንፕሮን የኬሌሽን መጠን ለመወሰን የሚረዱ ዘዴዎች |
የደረጃ ተቀባይነት፡ Q/320205 KAVNO7-2016
1. ሬጀንቶች እና ቁሳቁሶች
ሀ) ግላሲያል አሴቲክ አሲድ፡ በትንታኔያዊ መልኩ ንፁህ፤ ለ) ፐርክሎሪክ አሲድ፡ 0.0500ሞል/ሊ፤ ሐ) አመልካች፡ 0.1% የክሪስታል ቫዮሌት አመልካች (ግላሲያል አሴቲክ አሲድ)
2. ነፃ የአሚኖ አሲዶችን መወሰን
2.1 ናሙናዎቹ በ80°ሴ ለ1 ሰዓት ደርቀዋል።
2.2 ናሙናውን ወደ ክፍል የሙቀት መጠን በተፈጥሮ እንዲቀዘቅዝ ወይም ጥቅም ላይ ሊውል ወደሚችል የሙቀት መጠን እንዲቀዘቅዝ በደረቅ መያዣ ውስጥ ያስቀምጡት።
2.3 በግምት 0.1 ግራም የናሙና ናሙና (እስከ 0.001 ግራም ትክክለኛ) ወደ 250 ሚሊ ሊትር ደረቅ ሾጣጣ ብልቃጥ ይመዝኑ
2.4 ናሙናው የአካባቢውን እርጥበት እንዳይስብ ለማድረግ ወደሚቀጥለው ደረጃ በፍጥነት ይሂዱ።
2.5 25 ሚሊ ሊትር የግላሲያል አሴቲክ አሲድ ይጨምሩ እና ከ 5 ደቂቃዎች በማይበልጥ ጊዜ ውስጥ በደንብ ይቀላቅሉ።
2.6 2 የክሪስታል ቫዮሌት አመልካች ጠብታዎችን ይጨምሩ።
2.7 እንደ የመጨረሻ ነጥብ ቀለሙን ሳይቀይሩ መፍትሄው ከሐምራዊ ወደ አረንጓዴ ለ15 ሰከንዶች እስኪለወጥ ድረስ በ0.0500mol/L (±0.001) መደበኛ የፔርክሎሪክ አሲድ የቲትሬሽን መፍትሄ ቲትሬት ያድርጉ።
2.8 የተጠቀመውን መደበኛ መፍትሄ መጠን ይመዝግቡ።
2.9 ባዶውን ፈተና በተመሳሳይ ጊዜ ያካሂዱ።
- 3. ስሌት እና ውጤቶች
- ካታሊያን
- Physicochemical parameters
ቪ1 - በመደበኛ የፐርክሎሪክ አሲድ መፍትሄ፣ በሚሊሊየር (mL) ውስጥ ናሙናዎችን ለቲትሬሽን የሚያገለግል መጠን።
ቮ - ለቲትሬሽን ባዶነት ከመደበኛ የፐርክሎሪክ አሲድ መፍትሄ ጋር፣ በሚሊሊየር (mL) ውስጥ ጥቅም ላይ የሚውል መጠን፤
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
አድራሻ፡ ቁጥር 147 የኪንግፑ መንገድ፣ ሹዋን ታውን፣ ፑጂያንግ ካውንቲ፣ ቼንግዱ ከተማ፣ ሲቹዋን ግዛት፣ ቻይና
ስልክ: 86-18880477902
ምርቶች
ኦርጋኒክ ያልሆኑ ጥቃቅን ማዕድናት
- ኦርጋኒክ መከታተያ ማዕድናት
- ስዋሕሊ
- ብጁ አገልግሎት
- ፈጣን አገናኞች
የኩባንያ መገለጫ
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| ጉጅራቲ | ለጥያቄው ጠቅ ያድርጉ | © የቅጂ መብት - 2010-2025፡ መብቱ በህግ የተጠበቀ ነው። | የጣቢያ ካርታ ከፍተኛ ፍለጋ ስልክ |
| ስልክ | 86-18880477902 | ጃቫኒስ | ኢሜይል ዋትስአፕ |
| 8618880477902 | ቻይንኛ | ፈረንሳይኛ | |
| Bird | ቻይንኛ | ፈረንሳይኛ | ጀርመንኛ ስፓንኛ |
| Aquatic animals | ጃፓንኛ | ኮሪያኛ | አረብኛ ግሪክኛ |
| ቱሪክሽ | ጣሊያንኛ | ||
| Ruminant animal g/head day | January 0.75 | ኢንዶኔዥያን አፍሪካንስ ስዊድንኛ |
ፖሊሽ
- ባስክ
- ካታሊያን
- Physicochemical parameters
ሂንዲ
ላኦ
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
ሾና
ቡልጋርያኛ
- ሴቡዋኖ
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- ክሮኤሽያን
ደች
| Application object | ኡርዱ ቪትናሜሴ | Content in full-value feed (mg/kg) | Efficacy |
| ጉጅራቲ | የሄይቲ | ሃውሳ | ኪንያርዋንዳ ሕሞንግ ሃንጋሪያን |
| Piglets and fattening pigs | ኢግቦኛ | ጃቫኒስ | ካናዳ ክመር ኩርዲሽ |
| ክይርግያዝ | ላቲን | ||
| Bird | 300～400 | 45～60 | ማስዶንያን ማላይ ማላያላም |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
ኖርወይኛ
- ፓሽቶ
- Appearance: brownish-yellow granules
- Physicochemical parameters
ሰሪቢያን
ሴሶቶ
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
ሾና
ስንድሂ
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
ስዋሕሊ
ታጂክኛ
ታሚል
ተሉጉ
ታይ
| Application object | ኡርዱ ቪትናሜሴ | Content in full-value feed (mg/kg) | Efficacy |
| ዪዲሽ | ዮሩባ | ዙሉ | ኪንያርዋንዳ ኦሪያ ቱሪክሜን |
| ኡይግሁር | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
